human il-15r alpha antibody Search Results


antibodies against il 15ra  (R&D Systems)
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R&D Systems antibodies against il 15ra
Figure 3. Soluble IL-15 triggers a differential cell signal in RCC and RPTEC. A) Scatchard’s plot analysis: Effects of anti-IL-15Rb and cc mAbs on IL-15 binding to RCC. For the IL-15 binding experiments, RCC7 cells were incubated with increasing concentrations of radioiodinated rIL-15 in presence or not of the following neutralizing mAbs: anti-IL-2Rb and IL-2Rc. The nonspecific cell binding was determined in the presence of radioiodinated rhIL-15 and a 100-fold excess of unlabeled rhIL-15. Cell-bound (B) and unbound (free, F) fractions were measured, and the specific bound fraction was calculated by subtracting the nonspecific binding from the cell-bound fraction. On the ordinate is plotted the ratio of the specific bound fraction (expressed in sites per cell) over the total concentration (bound plus free) of radioiodinated rIL-15 (expressed in pM). On the abscissa bound fraction (expressed in sites per cell). The high affinity specific IL-15 binding (Kd = 375 pM, 413 IL-15 binding sites per cell), which was completely abrogated by neutralizing antibody against the IL-2Rb (inset) but not the cc chain, suggested the presence on RCC of an <t>IL-15Ra/IL-2Rb</t> complex. B) Detection of IL-15Rab complex by immunoprecipitation (IP) with anti-IL-15Ra (M161) or mouse IgG protein G-Sepharose-conjugate on
Antibodies Against Il 15ra, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human il 15rα affinity purified polyclonal antibody  (R&D Systems)
90
R&D Systems human il 15rα affinity purified polyclonal antibody
Figure 3. Soluble IL-15 triggers a differential cell signal in RCC and RPTEC. A) Scatchard’s plot analysis: Effects of anti-IL-15Rb and cc mAbs on IL-15 binding to RCC. For the IL-15 binding experiments, RCC7 cells were incubated with increasing concentrations of radioiodinated rIL-15 in presence or not of the following neutralizing mAbs: anti-IL-2Rb and IL-2Rc. The nonspecific cell binding was determined in the presence of radioiodinated rhIL-15 and a 100-fold excess of unlabeled rhIL-15. Cell-bound (B) and unbound (free, F) fractions were measured, and the specific bound fraction was calculated by subtracting the nonspecific binding from the cell-bound fraction. On the ordinate is plotted the ratio of the specific bound fraction (expressed in sites per cell) over the total concentration (bound plus free) of radioiodinated rIL-15 (expressed in pM). On the abscissa bound fraction (expressed in sites per cell). The high affinity specific IL-15 binding (Kd = 375 pM, 413 IL-15 binding sites per cell), which was completely abrogated by neutralizing antibody against the IL-2Rb (inset) but not the cc chain, suggested the presence on RCC of an <t>IL-15Ra/IL-2Rb</t> complex. B) Detection of IL-15Rab complex by immunoprecipitation (IP) with anti-IL-15Ra (M161) or mouse IgG protein G-Sepharose-conjugate on
Human Il 15rα Affinity Purified Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 15 super agonist  (R&D Systems)
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Figure 3. Soluble IL-15 triggers a differential cell signal in RCC and RPTEC. A) Scatchard’s plot analysis: Effects of anti-IL-15Rb and cc mAbs on IL-15 binding to RCC. For the IL-15 binding experiments, RCC7 cells were incubated with increasing concentrations of radioiodinated rIL-15 in presence or not of the following neutralizing mAbs: anti-IL-2Rb and IL-2Rc. The nonspecific cell binding was determined in the presence of radioiodinated rhIL-15 and a 100-fold excess of unlabeled rhIL-15. Cell-bound (B) and unbound (free, F) fractions were measured, and the specific bound fraction was calculated by subtracting the nonspecific binding from the cell-bound fraction. On the ordinate is plotted the ratio of the specific bound fraction (expressed in sites per cell) over the total concentration (bound plus free) of radioiodinated rIL-15 (expressed in pM). On the abscissa bound fraction (expressed in sites per cell). The high affinity specific IL-15 binding (Kd = 375 pM, 413 IL-15 binding sites per cell), which was completely abrogated by neutralizing antibody against the IL-2Rb (inset) but not the cc chain, suggested the presence on RCC of an <t>IL-15Ra/IL-2Rb</t> complex. B) Detection of IL-15Rab complex by immunoprecipitation (IP) with anti-IL-15Ra (M161) or mouse IgG protein G-Sepharose-conjugate on
Il 15 Super Agonist, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human il 15rα antibody  (R&D Systems)
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R&D Systems anti human il 15rα antibody
Figure 3. Soluble IL-15 triggers a differential cell signal in RCC and RPTEC. A) Scatchard’s plot analysis: Effects of anti-IL-15Rb and cc mAbs on IL-15 binding to RCC. For the IL-15 binding experiments, RCC7 cells were incubated with increasing concentrations of radioiodinated rIL-15 in presence or not of the following neutralizing mAbs: anti-IL-2Rb and IL-2Rc. The nonspecific cell binding was determined in the presence of radioiodinated rhIL-15 and a 100-fold excess of unlabeled rhIL-15. Cell-bound (B) and unbound (free, F) fractions were measured, and the specific bound fraction was calculated by subtracting the nonspecific binding from the cell-bound fraction. On the ordinate is plotted the ratio of the specific bound fraction (expressed in sites per cell) over the total concentration (bound plus free) of radioiodinated rIL-15 (expressed in pM). On the abscissa bound fraction (expressed in sites per cell). The high affinity specific IL-15 binding (Kd = 375 pM, 413 IL-15 binding sites per cell), which was completely abrogated by neutralizing antibody against the IL-2Rb (inset) but not the cc chain, suggested the presence on RCC of an <t>IL-15Ra/IL-2Rb</t> complex. B) Detection of IL-15Rab complex by immunoprecipitation (IP) with anti-IL-15Ra (M161) or mouse IgG protein G-Sepharose-conjugate on
Anti Human Il 15rα Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human il 15r alpha  (R&D Systems)
91
R&D Systems anti human il 15r alpha
Figure 3. Soluble IL-15 triggers a differential cell signal in RCC and RPTEC. A) Scatchard’s plot analysis: Effects of anti-IL-15Rb and cc mAbs on IL-15 binding to RCC. For the IL-15 binding experiments, RCC7 cells were incubated with increasing concentrations of radioiodinated rIL-15 in presence or not of the following neutralizing mAbs: anti-IL-2Rb and IL-2Rc. The nonspecific cell binding was determined in the presence of radioiodinated rhIL-15 and a 100-fold excess of unlabeled rhIL-15. Cell-bound (B) and unbound (free, F) fractions were measured, and the specific bound fraction was calculated by subtracting the nonspecific binding from the cell-bound fraction. On the ordinate is plotted the ratio of the specific bound fraction (expressed in sites per cell) over the total concentration (bound plus free) of radioiodinated rIL-15 (expressed in pM). On the abscissa bound fraction (expressed in sites per cell). The high affinity specific IL-15 binding (Kd = 375 pM, 413 IL-15 binding sites per cell), which was completely abrogated by neutralizing antibody against the IL-2Rb (inset) but not the cc chain, suggested the presence on RCC of an <t>IL-15Ra/IL-2Rb</t> complex. B) Detection of IL-15Rab complex by immunoprecipitation (IP) with anti-IL-15Ra (M161) or mouse IgG protein G-Sepharose-conjugate on
Anti Human Il 15r Alpha, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il15ra  (R&D Systems)
93
R&D Systems il15ra
Figure 3. Soluble IL-15 triggers a differential cell signal in RCC and RPTEC. A) Scatchard’s plot analysis: Effects of anti-IL-15Rb and cc mAbs on IL-15 binding to RCC. For the IL-15 binding experiments, RCC7 cells were incubated with increasing concentrations of radioiodinated rIL-15 in presence or not of the following neutralizing mAbs: anti-IL-2Rb and IL-2Rc. The nonspecific cell binding was determined in the presence of radioiodinated rhIL-15 and a 100-fold excess of unlabeled rhIL-15. Cell-bound (B) and unbound (free, F) fractions were measured, and the specific bound fraction was calculated by subtracting the nonspecific binding from the cell-bound fraction. On the ordinate is plotted the ratio of the specific bound fraction (expressed in sites per cell) over the total concentration (bound plus free) of radioiodinated rIL-15 (expressed in pM). On the abscissa bound fraction (expressed in sites per cell). The high affinity specific IL-15 binding (Kd = 375 pM, 413 IL-15 binding sites per cell), which was completely abrogated by neutralizing antibody against the IL-2Rb (inset) but not the cc chain, suggested the presence on RCC of an <t>IL-15Ra/IL-2Rb</t> complex. B) Detection of IL-15Rab complex by immunoprecipitation (IP) with anti-IL-15Ra (M161) or mouse IgG protein G-Sepharose-conjugate on
Il15ra, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti il 15rα antibodies  (R&D Systems)
86
R&D Systems anti il 15rα antibodies
Figure 3. Soluble IL-15 triggers a differential cell signal in RCC and RPTEC. A) Scatchard’s plot analysis: Effects of anti-IL-15Rb and cc mAbs on IL-15 binding to RCC. For the IL-15 binding experiments, RCC7 cells were incubated with increasing concentrations of radioiodinated rIL-15 in presence or not of the following neutralizing mAbs: anti-IL-2Rb and IL-2Rc. The nonspecific cell binding was determined in the presence of radioiodinated rhIL-15 and a 100-fold excess of unlabeled rhIL-15. Cell-bound (B) and unbound (free, F) fractions were measured, and the specific bound fraction was calculated by subtracting the nonspecific binding from the cell-bound fraction. On the ordinate is plotted the ratio of the specific bound fraction (expressed in sites per cell) over the total concentration (bound plus free) of radioiodinated rIL-15 (expressed in pM). On the abscissa bound fraction (expressed in sites per cell). The high affinity specific IL-15 binding (Kd = 375 pM, 413 IL-15 binding sites per cell), which was completely abrogated by neutralizing antibody against the IL-2Rb (inset) but not the cc chain, suggested the presence on RCC of an <t>IL-15Ra/IL-2Rb</t> complex. B) Detection of IL-15Rab complex by immunoprecipitation (IP) with anti-IL-15Ra (M161) or mouse IgG protein G-Sepharose-conjugate on
Anti Il 15rα Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine il 15rα  (R&D Systems)
86
R&D Systems murine il 15rα
Figure 3. Soluble IL-15 triggers a differential cell signal in RCC and RPTEC. A) Scatchard’s plot analysis: Effects of anti-IL-15Rb and cc mAbs on IL-15 binding to RCC. For the IL-15 binding experiments, RCC7 cells were incubated with increasing concentrations of radioiodinated rIL-15 in presence or not of the following neutralizing mAbs: anti-IL-2Rb and IL-2Rc. The nonspecific cell binding was determined in the presence of radioiodinated rhIL-15 and a 100-fold excess of unlabeled rhIL-15. Cell-bound (B) and unbound (free, F) fractions were measured, and the specific bound fraction was calculated by subtracting the nonspecific binding from the cell-bound fraction. On the ordinate is plotted the ratio of the specific bound fraction (expressed in sites per cell) over the total concentration (bound plus free) of radioiodinated rIL-15 (expressed in pM). On the abscissa bound fraction (expressed in sites per cell). The high affinity specific IL-15 binding (Kd = 375 pM, 413 IL-15 binding sites per cell), which was completely abrogated by neutralizing antibody against the IL-2Rb (inset) but not the cc chain, suggested the presence on RCC of an <t>IL-15Ra/IL-2Rb</t> complex. B) Detection of IL-15Rab complex by immunoprecipitation (IP) with anti-IL-15Ra (M161) or mouse IgG protein G-Sepharose-conjugate on
Murine Il 15rα, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3. Soluble IL-15 triggers a differential cell signal in RCC and RPTEC. A) Scatchard’s plot analysis: Effects of anti-IL-15Rb and cc mAbs on IL-15 binding to RCC. For the IL-15 binding experiments, RCC7 cells were incubated with increasing concentrations of radioiodinated rIL-15 in presence or not of the following neutralizing mAbs: anti-IL-2Rb and IL-2Rc. The nonspecific cell binding was determined in the presence of radioiodinated rhIL-15 and a 100-fold excess of unlabeled rhIL-15. Cell-bound (B) and unbound (free, F) fractions were measured, and the specific bound fraction was calculated by subtracting the nonspecific binding from the cell-bound fraction. On the ordinate is plotted the ratio of the specific bound fraction (expressed in sites per cell) over the total concentration (bound plus free) of radioiodinated rIL-15 (expressed in pM). On the abscissa bound fraction (expressed in sites per cell). The high affinity specific IL-15 binding (Kd = 375 pM, 413 IL-15 binding sites per cell), which was completely abrogated by neutralizing antibody against the IL-2Rb (inset) but not the cc chain, suggested the presence on RCC of an IL-15Ra/IL-2Rb complex. B) Detection of IL-15Rab complex by immunoprecipitation (IP) with anti-IL-15Ra (M161) or mouse IgG protein G-Sepharose-conjugate on

Journal: PloS one

Article Title: Interleukin-15 plays a central role in human kidney physiology and cancer through the γc signaling pathway.

doi: 10.1371/journal.pone.0031624

Figure Lengend Snippet: Figure 3. Soluble IL-15 triggers a differential cell signal in RCC and RPTEC. A) Scatchard’s plot analysis: Effects of anti-IL-15Rb and cc mAbs on IL-15 binding to RCC. For the IL-15 binding experiments, RCC7 cells were incubated with increasing concentrations of radioiodinated rIL-15 in presence or not of the following neutralizing mAbs: anti-IL-2Rb and IL-2Rc. The nonspecific cell binding was determined in the presence of radioiodinated rhIL-15 and a 100-fold excess of unlabeled rhIL-15. Cell-bound (B) and unbound (free, F) fractions were measured, and the specific bound fraction was calculated by subtracting the nonspecific binding from the cell-bound fraction. On the ordinate is plotted the ratio of the specific bound fraction (expressed in sites per cell) over the total concentration (bound plus free) of radioiodinated rIL-15 (expressed in pM). On the abscissa bound fraction (expressed in sites per cell). The high affinity specific IL-15 binding (Kd = 375 pM, 413 IL-15 binding sites per cell), which was completely abrogated by neutralizing antibody against the IL-2Rb (inset) but not the cc chain, suggested the presence on RCC of an IL-15Ra/IL-2Rb complex. B) Detection of IL-15Rab complex by immunoprecipitation (IP) with anti-IL-15Ra (M161) or mouse IgG protein G-Sepharose-conjugate on

Article Snippet: Antibodies against IL-15Ra (AF247), E-cadherin (AF648) and PE-conjugated antiE-cadherin (FAB18381P) were obtained from R&D Systems Europe Ltd (Abingdon, Oxon, U.K.), as well as neutralizing antiIL-2Rc (mAb2842) mAb.

Techniques: Binding Assay, Incubation, Concentration Assay, Immunoprecipitation